Heat-lamp-for-mouse-tail-vein-injection, braintree scientific, inc. po box 850498, braintree, ma 02185: tel. 781-917-9526: fax 978-244-8917. Tail vein injections in the mouse and rat sop page 3 of 3 5. restrain the tail while occluding the vein with your non‐dominant hand., use heat to dilate the tail veins which run laterally along each side of the tail. 3. clean the tail with 70% alcohol. 4.. Warming the mouse under a heat lamp helps to bring up the tail vein, but be careful not to heat stress the whole box as this may affect your results., warming the mouse (or tail only) causes vasodilation and provides better vein visibility. this may be done using a heating chamber, heating pad or a heating lamp, or dipping tail in warm water..
Insert the needle (small gauge, 27-30 for mice and 25-27 for rats), bevel up, into the vein towards the direction of the head. keep the needle and syringe parallel to the tail. aspiration is not advised as it may cause the vein to collapse, but a flash of blood in the hub of the needle may be seen when first placed., mission bio braintree scientific sells a plexiglas device that restrains the mouse with just its tail outside in the proper orientation for iv injection. you can easily modify the device with....
I've been attempting tail vein injections (50 ul of pbs containing a known concentration of a bacterial pathogen) for the past few weeks and have found that i am not as consistent as i would like to be in regards to injecting into a vein rather than into the peripheral fatty tissue., it is common practice to stimulate dilation of the tail veins in mice either by placing them in a warmer environment (e.g. at 28-30 ºc) for up to 30 minutes, or by placing the tail in warm (30-35 ºc) water. if a warming box or incubator is used, its temperature should be monitored carefully..
Nudes and white mice (fvb, balbc) are easier to inject than darker mice (c3h, c57) due to hair color and vein size, so if you're having an issue with dark mice you are not alone. what helped me was to use bright lights (better vein visibility) and heat them up for at least 15 minutes (not intense light, you don't want them to get sweaty).